Fig. 6: ONECUT2 is crucial for FGF2-induced HCC metastasis. | Cell Death & Disease

Fig. 6: ONECUT2 is crucial for FGF2-induced HCC metastasis.

From: ONECUT2 facilitates hepatocellular carcinoma metastasis by transcriptionally upregulating FGF2 and ACLY

Fig. 6

A Protein levels of ONECUT2 were measured in PLC/PRF/5 cells after ONECUT2 knockdown and FGF2 treatment (20 ng/ml, 24 h) by western blotting. B Transwell assays were used to evaluate migration and invasion of PLC/PRF/5 cells after ONECUT2 knockdown and FGF2 treatment (20 ng/ml, 24 h). C Protein levels of FGF2 and ONECUT2 were measured in PLC/PRF/5 cells after FGF2 overexpression and ONECUT2 knockdown by western blotting. D Transwell assays were used to evaluate migration and invasion of PLC/PRF/5 cells after FGF2 overexpression and ONECUT2 knockdown. E–J ONECUT2 knockdown inhibited FGF2-enhanced HCC metastasis. Representative bioluminescent imaging (E), bioluminescent signals (F), the incidence of pulmonary metastasis (G), number of pulmonary metastasis foci (H), overall survival (I), and H&E staining of the lungs (J) among four groups with ten mice in each group 9 weeks after orthotopic implantation of the indicated cells. Data are mean ± SD. *P < 0.05, **P < 0.01.

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