Fig. 2: FUT8 expression is negatively correlated with OS oncogenesis in vitro and in vivo. | Cell Death & Disease

Fig. 2: FUT8 expression is negatively correlated with OS oncogenesis in vitro and in vivo.

From: Alpha-(1,6)-fucosyltransferase (FUT8) affects the survival strategy of osteosarcoma by remodeling TNF/NF-κB2 signaling

Fig. 2

A, C Mean ± SD (N = 4) cell index, a measure of cell attachment and cell proliferation in vitro, for two FUT8-regulated OS cell lines (MNNG-F8, MNNG-siF8) and their control cell lines (F8Control, siControl, respectively) over time. RTCA assay confirmed that cell proliferation is enhanced in vitro in MNNG-siF8 cells, while it is reduced in MNNG-F8 cells compared to control cells. B, D Mean ± SD (N = 4) cell index at 48 h, 72 h, 96 h, 120 h. E, G Representative light microscopic images of PFA-fixed MNNG-F8, MNNG-siF8, and control cells stained with crystal violet, and (F, H) quantitation of colony counts after 2 weeks of in vitro maintenance, data were shown as mean ± SD (N = 3). I, K Images of excised flank tumors after injecting 200 μl of cell suspension from MNNG-F8 (N = 5), MNNG-siF8 (N = 5), or control cells (N = 5) in the nude mouse subcutaneous tumor model, and (J, L) quantitation of tumor volume (mean ± SD). *P < 0.05; **P < 0.01; ***P < 0.001.

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