Fig. 5: Apoptosis in FUT8-regulated OS cells occurs through a mitochondria-dependent pathway. | Cell Death & Disease

Fig. 5: Apoptosis in FUT8-regulated OS cells occurs through a mitochondria-dependent pathway.

From: Alpha-(1,6)-fucosyltransferase (FUT8) affects the survival strategy of osteosarcoma by remodeling TNF/NF-κB2 signaling

Fig. 5

A Representative immunofluorescent images of JC-1 staining in the four OS cell lines after treatment with 0.05 μM STS for 24 h. High ratios of red/green fluorescence represented a high mitochondrial transmembrane potentials (ΔΨM) while low ratios of red/green fluorescence represented a low ΔΨM (Scale bar: 25 μm). B Mean ± SD (N = 3) ratios of JC-1 red/green fluorescence. C Western blot of mitochondria-dependent apoptosis-related proteins (Bcl-2, Bcl-xl, Bfl-1, cleaved-caspase 8, and cleaved-caspase 9) confirmed that, compared to control cells, anti-apoptosis proteins were less abundant in MNNG-F8 cells but were more abundant in MNNG-siF8 cells. Relative expression was normalized to GAPDH. D Quantitation of relative expression (mean ± SD, N = 3) of mitochondria-dependent apoptosis-related proteins. ns, På 0.05; *P < 0.05; **P < 0.01.

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