Fig. 6: Knockdown of FUT8 in OS cells in vitro activates TNF/NF-κB2 signaling pathway. | Cell Death & Disease

Fig. 6: Knockdown of FUT8 in OS cells in vitro activates TNF/NF-κB2 signaling pathway.

From: Alpha-(1,6)-fucosyltransferase (FUT8) affects the survival strategy of osteosarcoma by remodeling TNF/NF-κB2 signaling

Fig. 6

A Western blot of proteins in a NF-κB2 signaling pathway show that this pathway was activated in MNNG-siF8 cells but inhibited in MNNG-F8 cells. B Analysis of p52 translocation in OS cell lines. Western blot probed for both in nuclear p52 and cytoplasmic p52. C Quantitation of ratio of nuclear p52 to cytoplasmic p52 expression. Mean ± SD (N = 3) ratio expression in MNNG-siF8 cells was higher, compared to controls, and lower in MNNG-F8 cells. Relative expression of nuclear p52 was normalized to PCNA while relative expression of cytoplasmic p52 was normalized to β-tubulin. D Representative p52 immunofluorescent images of cultured OS cell lines showed translocation of p52 into the nucleus. Red fluorescence represented nuclei, green fluorescence represented p52 (Scale bar: 25 μm). The translocation of p52 into the nuclei appeared visually to be enhanced in MNNG-siF8 cells but reduced in MNNG-F8 cells compared to control cells. E Quantitation of ratio of nuclear p52 to cytoplasmic p52 in cultured OS cell lines. Mean ± SD (N = 3) ratio of nuclear p52 fluorescence to cytoplasmic p52 fluorescence. *P < 0.05; **P < 0.01; ***P < 0.001.

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