Fig. 4: Treatment with SB216763 reduces the nuclear GR localization following Dexa treatment. | Cell Death & Disease

Fig. 4: Treatment with SB216763 reduces the nuclear GR localization following Dexa treatment.

From: Underlying mechanisms of glucocorticoid-induced β-cell death and dysfunction: a new role for glycogen synthase kinase 3

Fig. 4

A Representative Western Blot (left) and quantification (right) of nuclear and cytoplasmic GR protein levels in INS-1 832/13 after 3 h incubation with or without Dexamethasone (Dexa) and with or without SB216763 (SB) (n = 5). Tubulin is used as the marker of the cytoplasmic fraction. Histone H3 is used as the marker of the nuclear fraction. Results are expressed as the percentage of Dexa condition for each independent experiment. B Representative images showing DAPI (upper panel) and GR staining (lower panel) in INS-1 832/13 after 3 h incubation with or without Dexamethasone (Dexa) and 3 h with or without SB216763 (SB). White arrows show examples of nuclear staining of the GR. Scale bar: 20 μm. C Representative Western Blot (left) and quantification (right) of nuclear and cytoplasmic GSK3β protein level in INS-1 832/13 after 3 h incubation with or without Dexamethasone (Dexa) and with or without SB216763 (SB) (n = 5). Tubulin is used as the marker of the cytoplasmic fraction. Histone H3 is used as the marker of the nuclear fraction. Results are expressed as the percentage of NT condition for each independent experiment. D Representative Western Blot (left) and quantification (right) of nuclear and cytoplasmic GSK3α levels in INS-1 832/13 after 3 h incubation with or without Dexamethasone (Dexa) and with or without SB216763 (SB) (n = 5). Tubulin is used as the marker of the cytoplasmic fraction. Histone H3 is used as the marker of the nuclear fraction. Results are expressed as the percentage of NT condition for each independent experiment. Results are shown as means ± S.E.M. n represents the number of independent experiments. **p < 0.01.

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