Fig. 5: Treatment with LiCl or SB216763 reduces the GR expression in INS-1 832/13 cells as well as in rat and human islets. | Cell Death & Disease

Fig. 5: Treatment with LiCl or SB216763 reduces the GR expression in INS-1 832/13 cells as well as in rat and human islets.

From: Underlying mechanisms of glucocorticoid-induced β-cell death and dysfunction: a new role for glycogen synthase kinase 3

Fig. 5

A Relative mRNA level of GR, measured by qPCR (n = 4), in INS-1 832/13, after 24 h treatment with or without Dexamethasone (Dexa) and 27 h with or without SB216763 (SB). Results are expressed as percentage of NT condition for each independent experiment. B Representative Western Blot (right) and quantification (left) of GR protein level in INS-1 832/13 after 24 h incubation with or without Dexamethasone (Dexa) and 27 h incubation with or without SB216763 (SB) (n = 5). β-actin is used as loading control. Results are expressed as percentage of NT condition for each independent experiment. C Relative mRNA level of GR, measured by qPCR (n = 3) in rat islets, after 24 h incubation with or without Dexamethasone (Dexa) and 27 h incubation with or without LiCl or SB216763. Results are expressed as percentage of NT condition for each independent experiment. D Representative Western Blot (right) and quantification (left) of GR protein level in rat islets after 24 h incubation with or without Dexamethasone (Dexa) and 27 h incubation with or without LiCl (n = 4). β-actin is used as loading control. Results are expressed as percentage of NT condition for each independent experiment. E Relative mRNA level of human GR, measured by qPCR (n = 4), in human islets, after 24 h treatment with or without LiCl. Results are expressed as percentage of NT condition for each independent experiment. Cyclophilin A is used as housekeeping gene in figure A and C. TBP is used as housekeeping gene in figure E.

Back to article page