Fig. 3: TTK inhibitors impede ovarian cancer proliferation in vitro. | Cell Death & Disease

Fig. 3: TTK inhibitors impede ovarian cancer proliferation in vitro.

From: TTK inhibition increases cisplatin sensitivity in high-grade serous ovarian carcinoma through the mTOR/autophagy pathway

Fig. 3

A CAOV3 and OV90 cells (3 × 103 cells per well of a 96-well plate) were incubated with a series of B389 concentrations (0, 0.1, 1, 5, 10, 50, 100, or 1000 nM) for 96 and 120 h, respectively. MTT assays were performed to determine the IC50 of B389 in CAOV3 (IC50 = 7.39 nM, 96 h) and OV90 (IC50 = 161.84 nM, 120 h) cells. B CAOV3 and OV90 cells were treated with different concentrations of B389 (0, 5, or 10 nM for CAOV3 cells and 0, 100, or 500 nM for OV90 cells) for 96 h. The dose-dependent effect of B389 on protein levels of TTK, H3, pH3 (ser10) and β-actin was detected using western blotting. C CAOV3 and OV90 cells (1.5 × 103 cells per well of a 96-well plate) were cultured with different concentrations of B389 (0, 1, 10, or 100 nM for CAOV3 cells and 0, 10, 100, or 500 nM for OV90 cells). Dose- and time-dependent effects of B389 on the proliferation of CAOV3 and OV90 cells were detected using MTT assays. D First, 1.5 × 103 cells were seeded in each well of a six-well plate, different concentrations of B389 (0, 10, or 100 nM for CAOV3 cells and 0, 100, or 500 nM for OV90 cells) were added after 10 days culture, and cells were treated for 4 days. The effect of B389 on the colony formation ability of ovarian cancer cells was detected. E. CAOV3 and OV90 cells were incubated with series concentrations of CFI (0, 0.1, 1, 5, 10, 50, 100, 1000, or 10,000 nM) for 96 h. MTT assays were performed to determine the IC50 of CFI in CAOV3 (IC50 = 39.77 nM) and OV90 (IC50 = 10111 nM) cells. F CAOV3 and OV90 cells were treated with different concentrations of CFI (0, 30, or 60 nM for CAOV3 cells and 0, 5, or 10 μM for OV90 cells) for 96 h. Levels of the TTK, H3, pH3 (ser10) and β-actin proteins were detected using western blotting after CFI treatment. G The antiproliferative effect of CFI (0, 5, 50, or 100 nM for CAOV3 cells and 0, 100, 1000, or 10,000 nM for OV90 cells) on CAOV3 and OV90 cells was detected using MTT assays. H CFI treatment (0, 30, or 60 nM for CAOV3 cells and 0, 5, or 10 μM for OV90 cells) inhibited the colony formation ability of ovarian cancer cells (data are mean ± SEM, *P < 0.05, **P < 0.01, n = 3).

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