Fig. 5: The effects of FDI-6 and/or Olaparib on cell cycle progression and genes involved in cell cycle control.

A The effects of FDI-6 and/or Olaparib on cell cycle progression in MDA-MB-231 cells. B The effects of FOXM1 shRNA and/or Olaparib on cell cycle progression in MDA-MB-231 cells. Relative expression of genes involved in cell cycle control analyzed by Q-PCR in MDA-MB-231 cells (C) and MDA-MB-231 xenografts (D). E The effects of FDI-6 and/or Olaparib on the expression of proteins involved in cell cycle control analyzed by Western blots in MDA-MB-231 cells. F The effects of FOXM1 shRNA and/or Olaparib on the expression of proteins involved in cell cycle control analyzed by Western blots in MDA-MB-231 cells. G The effects of FDI-6 and/or Olaparib on the expression of proteins involved in cell cycle control analyzed by Western blots in vivo. The results from three independent experiments were statistically analyzed using one-way ANOVA: *P < 0.05, **P < 0.01 compared with the control; #P < 0.05, ##P < 0.01 compared with the FDI-6/Olaparib-combined group (FDI-6/Olaparib: 4.0 + 4.0 μM, FDI-6/Olaparib: 30 + 30 mg/kg).