Fig. 6: The effects of FDI-6 and/or Olaparib on DNA damage and genes involved in DNA repair. | Cell Death & Disease

Fig. 6: The effects of FDI-6 and/or Olaparib on DNA damage and genes involved in DNA repair.

From: FDI-6 inhibits the expression and function of FOXM1 to sensitize BRCA-proficient triple-negative breast cancer cells to Olaparib by regulating cell cycle progression and DNA damage repair

Fig. 6

A The effects of FDI-6 and/or Olaparib on DNA damage detected by alkaline comet assay. B The effects of FOXM1 shRNA and/or Olaparib on DNA damage detected by alkaline comet assay. C The expression of γh2AX analyzed by immunofluorescence. Green represents the cells stained with anti-γh2AX antibody, blue represents the nuclei stained with DAPI. D Mean intensity of immunofluorescence. The effects of FDI-6 and/or Olaparib on the expression of DNA repair-related factors analyzed by Q-PCR (E) and (F) in MDA-MB-231 cells. G The effects of FOXM1 shRNA and/or Olaparib on the expression of DNA repair-related proteins analyzed by Western blots. H Effects of FDI-6 and/or Olaparib on the expression of γh2AX and Rad51 in vivo analyzed by immunohistochemistry. I Percentage of immunohistochemically stained dots. Effects of FDI-6 and/or Olaparib on the expression of factors involved in DNA repair analyzed by Q-PCR (J) and Western blots (K). The results from three independent experiments were statistically analyzed using one-way ANOVA: *P < 0.05, **P < 0.01 compared with the control; #P < 0.05, ##P < 0.01 compared with the FDI-6/Olaparib-combined group (FDI-6/Olaparib: 4.0 + 4.0 μM, FDI-6/Olaparib: 30 + 30 mg/kg).

Back to article page