Fig. 3: Genome-wide identification and biological involvement analyses of miRNA targets during liver development. | Cell Death & Disease

Fig. 3: Genome-wide identification and biological involvement analyses of miRNA targets during liver development.

From: Genome-wide identification of microRNA targets reveals positive regulation of the Hippo pathway by miR-122 during liver development

Fig. 3

A Motif analysis of Ago2-bound mRNAs representative of the developmental stages of liver. Up panel: e12.5, bottom panel: adult. B Representative of enriched Gene ontology (GO) biological process terms of targets of five clusters miRNAs. FDR false discovery rate; percentage, number of genes involved in a particular term divided by total genes in this term. C Representative of enriched KEGG pathways of targets of five clusters miRNAs. D Mammalian Phenotype (MP) Ontology enrichment analysis of targets of top 5 development-downregulated miRNAs. E MP Ontology enrichment analysis of targets of top 5 development-upregulated miRNAs. F Gene expression differences assessed by microarray analysis of background genes and targets predicted by seed in the 3′UTR or miR-122 between WT and miR-122 KO mice. 3′UTR targets identified by Ago HITS-CLIP. The gene expression data of WT and miR-122 KO mice were reported by Hsu et al. [15] and obtained from the GEO dataset (GSE20610). p values were calculated with a one-sided Kolmogorov−Smirnov test. G Venn diagram showing the overlap between mouse miR-122 targets identified by Starbase and our HITS-CLIP (up panel). Overlap between experimentally validated mouse miR-122 targets and our HITS-CLIP (bottom panel). The experimentally validated mouse miR-122 targets were obtained by integrating miRecords [64] and miRTarBase [65], two widely used databases collecting miRNA targets reported in the literature. H KEGG analysis of miR-122 targets identified by our Ago HITS-CLIP.

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