Fig. 4: miR-122 positively regulates the Hippo pathway. | Cell Death & Disease

Fig. 4: miR-122 positively regulates the Hippo pathway.

From: Genome-wide identification of microRNA targets reveals positive regulation of the Hippo pathway by miR-122 during liver development

Fig. 4

A Structures of the inducible lentiviral plasmids expressing of miR-122. B Relative expression of miR-122 either after induced expression or transfection, values are mean ± SEM. C 46-pathway (Cignal 45-Pathway Reporter Array plus 8xGTIIC-luciferase Hippo pathway reporter) assays revealed the effects of miR-122 on the activity of various pathways in inducible Hepa1–6 cell lines. The data were log2-transformed, values are mean ± SEM. D 8xGTIIC-luciferase reporter assay of mimics transfection and inducible overexpression of miR-122; WT Hepa1–6 cells treated with doxycycline were also examined to rule out the influence of doxycycline, values are mean ± SEM. E 8xGTIIC-luciferase reporter assay after transfection of miR-122 mimics and inhibitors into BNL CL.2 cells, values are mean ± SEM. Analysis of the change of well-documented Hippo pathway downstream genes after either induced or mimics-transfection-mediated overexpression of miR-122 in Hepa1–6 cells (F) and BNL CL.2 cells (G), values are mean ± SEM. (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001).

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