Fig. 5: A number of miR-122 targets influence the Hippo pathway activity. | Cell Death & Disease

Fig. 5: A number of miR-122 targets influence the Hippo pathway activity.

From: Genome-wide identification of microRNA targets reveals positive regulation of the Hippo pathway by miR-122 during liver development

Fig. 5

A Veen diagram showing overlap between mouse miR-122 targets identified in this study and potential Hippo pathway regulators. The potential miR-122 targets were considered as Ago binding mRNA in 3′UTR, CDS with miR-122 seed sequences. B Network analysis of miR-122 targets that may serve as Hippo pathway regulators, the interactions between proteins were analyzed using string database. The miR-122 targets are indicated by red font, and the core component genes of Hippo pathway are indicated by black font. C miR-122 target wild-type dual-luciferase reporter assay, values are mean ± SEM. D miR-122 target mutant-type dual-luciferase reporter assay, values are mean ± SEM. E RT-qPCR showing the change of mRNA levels of targets after transfection of miR-122 mimics, values are mean ± SEM. F Hippo signaling pathway reporter assay after silencing potential miR-122 targets that may serve as regulator of Hippo pathway, values are mean ± SEM. (**p < 0.01; ***p < 0.001; ****p < 0.0001).

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