Fig. 6: miR-122 can directly target Taz and promotes phosphorylation of YAP by targeting Ppp1cc. | Cell Death & Disease

Fig. 6: miR-122 can directly target Taz and promotes phosphorylation of YAP by targeting Ppp1cc.

From: Genome-wide identification of microRNA targets reveals positive regulation of the Hippo pathway by miR-122 during liver development

Fig. 6

A Western blot showing the change of TAZ protein and YAP phosphorylated protein level during the mouse liver development. B Western blot showing the effect of overexpression of miR-122 on TAZ protein expression in Hepa1–6 and BNL CL.2 cells. C 8xGTIIC-luciferase reporter assay showing the effect of co-overexpression of miR-122 and Taz on the Hippo pathway, values are mean ± SEM. D Western blot showing the effect of overexpression of miR-122 on phosphorylation of YAP and the total YAP protein. E Western blot showing the effect of overexpression of miR-122 on PPP1CC protein expression in Hepa1–6 and BNL CL.2 cells. F Western blot showing the effect of knockdown (left) or overexpression (right) of Ppp1cc on phosphorylation of YAP. G 8xGTIIC-luciferase reporter assay showing the effect of co-overexpression of miR-122 and Ppp1cc on the Hippo pathway, values are mean ± SEM. H RT-qPCR showing the change of mRNA of Ppp1cc and hippo pathway downstream genes after silencing of Ppp1cc, values are mean ± SEM. (*p < 0.05; **p < 0.01; ***p < 0.001).

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