Fig. 4: TRIM21 induces TRPM2 ubiquitination at Lys1218. | Cell Death & Disease

Fig. 4: TRIM21 induces TRPM2 ubiquitination at Lys1218.

From: LncTRPM2-AS inhibits TRIM21-mediated TRPM2 ubiquitination and prevents autophagy-induced apoptosis of macrophages in asthma

Fig. 4

A Potential ubiquitination sites of TRPM2 obtained from http://plmd.biocuckoo.org/. B, C Western blot analysis of TRPM2 in HEK293T cells co-transfected with Flag-Trim21, HA-Ub, and wild type or mutant TRPM2 (TRPM2 mutants: K8R, K55R, K117R, K307R, K314R, K405R, K423R, K596R, K703R, K1218R, and K1544R). D Immunoprecipitation analysis of ubiquitination of TRPM2 in HEK293T cells co-transfected with HA-Ub, Flag-TRIM21, and TRPM2 (WT or K1218R). E RIP experiments were used to detect the amount of lncTRPM2-AS after IP TRPM2. F RIP experiments were used to detect the amount of lncTRPM2-AS after IP Myc which represent different domains of TRPM2 as indicated. Bars, ± SD; n = 3, ns not significant; ***P < 0.001.

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