Fig. 3: TS1 reduces activation and collagen expression of murine lung fibroblasts. | Cell Death & Disease

Fig. 3: TS1 reduces activation and collagen expression of murine lung fibroblasts.

From: Wet-dry-wet drug screen leads to the synthesis of TS1, a novel compound reversing lung fibrosis through inhibition of myofibroblast differentiation

Fig. 3

A Flowchart showing the 19 compounds structurally related to dopamine which were predicted by machine learning to reduce αSMA expression (yellow). CHEMBL145929 was selected as the representative molecule from which six novel derivatives were synthesized, TS1-TS6 (orange). B Murine lung fibroblasts from αSMA-RFP, COLL-EGFP mice were treated in vitro with TGFβ and lipophilic TS1-6. Representative images are shown (Hoechst in blue, COLL-EGFP in green, αSMA-RFP in red). C–E Quantification of the number of αSMA positive cells, collagen positive cells, and total cell number (shown as fold over control). F Murine lung fibroblasts from COLL-EGFP/αSMA-RFP mice were treated ex vivo with TGFβ and the hydrophilic TS1-HCl. Representative images are shown (Hoechst in blue, COLL-EGFP in green, αSMA-RFP in red). G–I Quantification of the number of αSMA positive cells, collagen positive cells, and total cell number (shown as fold over control). All data are shown as mean ± SD. Statistical significance was determined using a one-way ANOVA followed by Dunnett’s multiple comparison test, *P < 0.05, **P < 0.01, n = 3.

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