Fig. 3: MYH9 promoted proliferation, invasion, migration, and TMZ resistance in gliomas.

A, B MTT assays showed MYH9 downregulation observably inhibited the cell growth. C Edu incorporation assays showed knockdown of MYH9 decreased the cell proliferation by blocking G1/S phase transition in glioma cells. D Intracranial orthotopic transplantation suggested knockdown of MYH9 inhibited the tumorigenesis in gliomas. E, F Transwell (E) and Boyden (F) chamber assays confirmed knockdown of MYH9 inhibited the ability of cell invasion and migration. G, H Dose–response curves were delineated for TMZ treatment in shMYH9 cells and the control groups to identify the role of MYH9 in chemoresistance. I Western blot assay confirmed the change of the downstream pathway of MYH9.