Fig. 6: Effect of RIPK1 inhibition by RIPA56 in a murine model of ACLF.
From: The role of RIPK1 mediated cell death in acute on chronic liver failure
C57BL/6 mice were treated with CCl4 for 6 weeks and subsequently injected with GalN. All animals were sacrificed 48 h after GalN injection. A GalN injection was associated with a significant increase of ALT levels. B Caspase 3/7 and 8 expression showed a mixed response without proving significance. C TUNEL staining of liver tissue showed that GalN injection induced hepatocyte cell death with high expression of RIPK3 (magnification, 10x and 40x). RIPK1 inhibition by RIPA 56 significantly reduced ALT levels, abrogated tissue injury (TUNEL staining) and reduced the liver RIPK3, pMLK, cleaved-Caspase 3 and 8 expression. The optical density of immunostaining intensity (mean ± SEM) was measured. Data were analyzed using Student’s t test or Mann–Whitney U test. Only significant p values were displayed *p < 0.05, **p < 0.01, ***p < 0.001.
