Fig. 5: CD82 overexpression promoted axon regeneration, RGC survival, and visual function after optic nerve crush.
From: CD82 protects against glaucomatous axonal transport deficits via mTORC1 activation in mice
a Timeline of the major procedures for exploring optic nerve regeneration and RGC survival after ONC. b Representative images showing axonal regeneration of optic nerve 14 or 28 days after ONC with or without CD82 overexpression. The yellow dashed line indicating the distances from the crush site. Scale bar, 200 μm. (dpc = day post crush). c Statistical analysis of regenerated axons shown in (b), n = 4 optic nerves, one-way ANOVA, *p < 0.05 for CD82 + ONC 14dpc v.s ONC 14dpc and CD82 + ONC 28dpc v.s ONC 28dpc. d Immunostaining of Rbpms in retina flat mount to mark RGC somas in control group and 7 day-post-ONC groups with or without CD82 overexpression. Scale bar, 100 μm. e Schematic indicating observation area in retina flat mount. f Quantification of RGC numbers shown in (d). Four nonoverlapping fields of view chosen for analysis in each retina, n = 4 retinas, one-way ANOVA, ***P < 0.001. g Schematic drawing of mouse dark/light preference tests to evaluate light perception. h Statistical analysis of mouse dark/light preference tests. i Schematic drawing of mouse optomotor response tests to evaluate visual acuity. j Statistical analysis of mouse optomotor response. Statistical tests in (h) and (j) using one-way ANOVA, n = 20 mice, *P < 0.05, **P < 0.01, ***P < 0.001.
