Fig. 2: KDM4A-AS1 promotes HCC cell proliferation, migration, and invasion. | Cell Death & Disease

Fig. 2: KDM4A-AS1 promotes HCC cell proliferation, migration, and invasion.

From: HIF-1α-activated long non-coding RNA KDM4A-AS1 promotes hepatocellular carcinoma progression via the miR-411-5p/KPNA2/AKT pathway

Fig. 2

A Hep3B cells were transfected with a vector carrying KDM4A-AS1 or empty vector, and KDM4A-AS1 shRNA (sh-KDM4A-AS1#1 and sh-KDM4A-AS1#2) or nontargeting shRNA (sh-control) was transfected into SK-Hep-1 cells. CCK-8 assay was performed to determine the effect of KDM4A-AS1 overexpression or knockdown on HCC cell viability. B Colony formation assay was utilized to evaluate the function of KDM4A-AS1 in HCC cell proliferation. C Edu assay was carried out to explore the proliferation of HCC cells affected by KDM4A-AS1 overexpression or knockdown. Scale bar: 20 μm. D The effect of KDM4A-AS1 on the migration and invasion abilities of HCC cells was detected by the transwell assay. Scale bar: 100 μm. E E-cadherin, N-cadherin, and Vimentin levels were detected to identify the EMT process of HCC cells affected by KDM4A-AS1 overexpression or knockdown. All the data are presented as mean ± SD of at least three independent experiments. *P < 0.05.

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