Fig. 4: KDM4A-AS1 functions as a molecular sponge of miR-411-5p. | Cell Death & Disease

Fig. 4: KDM4A-AS1 functions as a molecular sponge of miR-411-5p.

From: HIF-1α-activated long non-coding RNA KDM4A-AS1 promotes hepatocellular carcinoma progression via the miR-411-5p/KPNA2/AKT pathway

Fig. 4

A A subcellular fractionation assay was conducted to investigate the localization of KDM4A-AS1 in HCC cells. B KDM4A-AS1 overexpression reduced and KDM4A-AS1 silencing increased miR-411-5p expression in HCC cells. miR-4529-5p, miR-4533, and miR-4673 levels were not affected by KDM4A-AS1 alteration. C The levels of miR-411-5p in HCC cell lines (Hep3B, Huh7, HepG2, HCCLM3, MHCC-97H, SK-Hep-1) and the normal hepatic cell line (MIHA). D miR-411-5p depletion increased and miR-411-5p overexpression reduced KDM4A-AS1 expression in HCC cells. E The predicted miR-411-5p-binding sites were mutated in KDM4A-AS1. The luciferase activity of HEK293T cells co-transfected with miR-411-5p mimics and wild-type (wt) or mutant (mt) KDM4A-AS1 was measured. F Anti-AGO2 RIP was performed in Hep3B cells transiently overexpressing miR-411-5p, and RT-qPCR was carried out to detect miR-411-5p and endogenous KDM4A-AS1 associated with AGO2. G KDM4A-AS1 level was determined in samples pulled down by biotinylated wt or mt miR-411-5p. All the data are presented as mean ± SD of at least three independent experiments. *P < 0.05.

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