Fig. 6: KPNA2 is a direct target of miR-411-5p in HCC cells. | Cell Death & Disease

Fig. 6: KPNA2 is a direct target of miR-411-5p in HCC cells.

From: HIF-1α-activated long non-coding RNA KDM4A-AS1 promotes hepatocellular carcinoma progression via the miR-411-5p/KPNA2/AKT pathway

Fig. 6

A Twenty-two potential targets of miR-411-5p were predicted in all three online bioinformatics tools. B miR-411-5p inversely regulated KPNA2 mRNA level rather than CD200 and SEMA3A in HCC cells. C miR-411-5p silencing upregulated and miR-411-5p overexpression increased KPNA2 protein level in HCC cells. D Complementary sequences between miR-411-5p and KPNA2 3’UTR were mutated, and HEK293T cells co-transfected with miR-411-5p mimics and wt or mt KPNA2 3’UTR was detected for luciferase activity. E Hep3B cells transfected with miR-411-5p mimics were subjected to anti-AGO2 RIP to detect RNAs associated with AGO2. F KPNA2 mRNA level was determined in samples pulled down by biotinylated wt or mt miR-411-5p. G Western blotting was performed to detect KPNA2 expression in HCC cells transfected with the indicating vectors. All the data are presented as mean ± SD of at least three independent experiments. *P < 0.05.

Back to article page