Fig. 1: TRPV1 is upregulated during neuroinflammation.
From: TRPV1 channel mediates NLRP3 inflammasome-dependent neuroinflammation in microglia
A–C Primary mouse microglia were challenged with 100 ng/ml LPS for 3 h. A mRNA of TRPV1 was assessed by qRT-PCR. B Immunostaining of TRPV1 was developed to check its expression and location change, and statistical results are shown in (C). D–F Immunostaining assay to check the expression of microglia TRPV1 in the spinal cord tissue of EAE mice. D Immunofluorescence staining of Iba-1 and TRPV1 in the spinal cord tissue of EAE mice, and statistical results are shown in (E). F Colocalization analysis of Iba-1 and TRPV1 in the spinal cord tissues of EAE mice. Representative images from n = 3 mice per group are displayed. Scale bar: 25μm. *p < 0.05, ***p < 0.001.
