Fig. 4: Autophagy degradation disorder after Sidt2 deletion in mouse kidney cells. | Cell Death & Disease

Fig. 4: Autophagy degradation disorder after Sidt2 deletion in mouse kidney cells.

From: Sidt2 is a key protein in the autophagy-lysosomal degradation pathway and is essential for the maintenance of kidney structure and filtration function

Fig. 4

A LC3-II and P62 expression by MPC5 cells incubated for 16 h with 0, 10, 25, 50, 100, or 200 μM CQ shown by western blot; B LC3-II statistical chart of MPC5 cells; C P62 statistical chart of MPC5 cells; D SV40 MES 13 cell incubation for 16 h with 0, 10, 25, 50, 100, or 200 μM CQ. Western blot shows LC3-II and P62 expression; E LC3-II statistical chart of SV40 MES 13 cells; F SV40 MES 13 cell P62 statistical chart; G determination whether CQ induces the expression of key autophagy proteins before and after Sidt2 knockout in MPC5 cells; H statistical chart of (G); I changes in the autophagy flux in MPC5 cells on Sidt2 knockout (LC3-II + CQ/β-Actin)/(LC3-II − CQ/β-Actin) (ref. [27]); J determination whether chloroquine induces the expression of key autophagy proteins before and after Sidt2 knockout in SV40 MES 13 cells; K statistical chart of (J); L changes of the autophagy flux in SV40 MES 13 cells on Sidt2 knockout (LC3-II + CQ/β-Actin)/(LC3-II − CQ/β-Actin) (ref. [27]). *P < 0.05, **P < 0.01, ***P < 0.001.

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