Fig. 5: Autophagosome and lysosome fusion is prevented after Sidt2 deletion.

A Immunofluorescence co-localization of LC3B and LAMP1 in MPC5 and SV40 MES 13 cells before and after Sidt2 knockout, analysis with Pearson’s correlation coefficient; B comparison of LC3B fluorescence points before and after Sidt2 knockout in MPC5 and SV40 MES 13 cells; C comparison of Pearson’s correlation coefficient before and after Sidt2 knockout in MPC5 and SV40 MES 13 cells; D comparison of the fusion rate before and after Sidt2 knockout in MPC5 and SV40 MES 13 cells (the ratio of the number of co-localized fluorescent spots of LAMP1 and LC3B to the number of fluorescent spots of LAMP1); *P < 0.05, **P < 0.01.