Fig. 2: Cdk5rap2an/an embryos with reduced body weight exhibit elevated SA-β-gal staining compared to Cdk5rap2+/an and Cdk5rap2+/+ embryos. | Cell Death & Disease

Fig. 2: Cdk5rap2an/an embryos with reduced body weight exhibit elevated SA-β-gal staining compared to Cdk5rap2+/an and Cdk5rap2+/+ embryos.

From: CDK5RAP2 loss-of-function causes premature cell senescence via the GSK3β/β-catenin-WIP1 pathway

Fig. 2

A Average weights of isolated E17.5 (left panel) and E12.5 to E17.5 (right panel). Cdk5rap2+/+, Cdk5rap2+/an, and Cdk5rap2an/an embryos from different litters are shown. B SA-β-gal staining of E12.5-E14.5 Cdk5rap2an/an and Cdk5rap2+/an and Cdk5rap2+/+ littermate embryos was performed as described in Materials and Methods. C MEFs isolated from Cdk5rap2+/+, Cdk5rap2+/an, and Cdk5rap2an/an embryos were subjected to SA-β-gal staining. Representative images (left panel) are from one of three independent experiments (n = 3) showing similar staining patterns. The number of SA-β-gal positive cells was assessed in ~200 cells per treatment group in each of the 3 independent experiments (n = 3). *p = 0.0002. D Growth of MEFs obtained from Cdk5rap2+/+, Cdk5rap2+/an, and Cdk5rap2an/an embryos were analyzed by cell viability assay.

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