Fig. 5: CDK5RAP2 loss reduces β-catenin level.
From: CDK5RAP2 loss-of-function causes premature cell senescence via the GSK3β/β-catenin-WIP1 pathway
A The WIP1 promoter contains an NF-κB binding site [32]. BJ cells transfected with CDK5RAP2 siRNA #2 (B), or β-catenin siRNA (C) for 3 days were analyzed by SDS-PAGE and immunoblotting. Nuclear fractions and whole-cell lysates in (B) were probed for β-catenin and histone H3 (nuclear fraction) or CDK5RAP2 and actin (whole cell lysate), and whole cell lysates in (C) were probed for β-catenin, WIP1, and actin. Actin blot was used as a loading control. Representative blots are from one of three independent experiments (n = 3) showing similar results. Ratios of levels of β-catenin vs histone H3 (in nuclear fraction) or actin (in whole cell lysate) in (B) and β-catenin or WIP1 vs actin in (C), right panels, and standard deviation for the 3 independent sets of experiments were calculated as described for CDK5RAP2 vs actin in Fig. 1, with values from cells transfected with control siRNA normalized to 1.0. *p < 0.05 (in B). *p < 0.005 (in C).
