Fig. 8: CDK5RAP2 regulates WIP1 promoter activity via β-catenin.
From: CDK5RAP2 loss-of-function causes premature cell senescence via the GSK3β/β-catenin-WIP1 pathway
A Sequence of pGL3 luciferase reporter vector carrying WIP1 or WIP1-ΔκB promoter. B, C Loss of CDK5RAP2 (B left and right panels) or β-catenin (C) inhibits WIP1 promoter activity as measured by luciferase reporter activity but inhibition of GSK3β with TWS119 (5 μM in DMSO) in CDK5RAP2-depleted cells restores WIP1 promoter activity (B, right panel). Data represent means ± SD from three (n = 3) independent experiments. *p < 0.05.
