Fig. 2: METTL14 depletion promotes CRC metastatic in vitro and in vivo. | Cell Death & Disease

Fig. 2: METTL14 depletion promotes CRC metastatic in vitro and in vivo.

From: TCF4 and HuR mediated-METTL14 suppresses dissemination of colorectal cancer via N6-methyladenosine-dependent silencing of ARRDC4

Fig. 2

The knockdown efficiency of METTL14 was confirmed by western blot (A) and qRT-PCR (B) after lentivirus infection in SW480 and HCT116 cells. C Total RNA isolated from METTL14-knockdown CRC cells were subjected to m6A dot blot assay. Methylene blue staining acted as a loading control. Representative images and quantification of transwell assay of METTL14-konckdown SW480 (D) and HCT116 cells (E). scale bars, 100 μm. F–H Xenografts derived from HCT116-shM14 or shCON cells (n = 4). Tumor volume was recorded at indicated time to establish a growth curve (G) and tumors weight (H) were measured after mice sacrificed. I–L Analysis of metastatic liver nodules after the tail vein injection of METTL14-knockdown or control HCT116 cells (n = 10 mice per group), evident metastatic lesions were indicated with yellow arrows. I Different groups of mice were subjected to magnetic resonance imaging (MRI) scans and the cross section drawing of mice were exhibited. L Hematoxylin-eosin staining (HE) of metastatic liver tissue. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

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