Fig. 8: TCF4 regulates METTL14 expression in CRC cells.

A Data from the UCSC genome bioinformatics site (http://genome.ucsc. edu/) showed high enrichment of H3K27ac in the promoter of METTL14. B METTL14 protein expression was determined by western blotting in SW480, HCT116 and HCT15 cells with C646 (20 μm) treatment. C METTL14 protein expression was determined by western blot in HCT116 and HCT15 cells with P300 knockdown. D Venn diagram shows the overlap of transcription factors of METTL14 predicted by PROMO and ChIPBase, respectively. E, F METTL14 protein expression was determined by western blotting in SW620 cells with CEBPB, ETS1, TCF4 knockdown, respectively. G Relative mRNA expression of METTL14 and TCF4 in SW620 cells with TCF4 knockdown were measured by qRT-PCR. H ChIP assay was performed in SW620 cells to determine whether TCF4 could bind to the promoter of METTL14. I SW620 cells were transfected with si-NC, siTCF4 or flag-ubiquitin. After METTL14 was immunoprecipitated from equal amounts of lysates, the ubiquitination of METTL14 was examined by western blot. J TCF4 expression in the GEPIA date-base. K The correlation of TCF4 and METTL14 expression in the GEPIA date-base.