Fig. 2: Knockdown of circPPP6R3 suppressed the proliferation, migration, and invasion of ccRCC cells.

A qRT-PCR detected the expression of circPPP6R3 and PPP6R3 mRNA in Caki-1 and ACHN cells after transfected with siRNAs targeting circPPP6R3. B–D MTS assay, EdU incorporation assay, and colony formation assay were employed to detect the proliferation of ccRCC cells. E, F Transwell assay, invasion assay, and wound-healing assay were performed to detect the migrative and invasive abilities of ccRCC cells.