Fig. 4: circPPP6R3 served as a sponge for miR-1238-3p. | Cell Death & Disease

Fig. 4: circPPP6R3 served as a sponge for miR-1238-3p.

From: Circular RNA circPPP6R3 upregulates CD44 to promote the progression of clear cell renal cell carcinoma via sponging miR-1238-3p

Fig. 4

A FISH experiment confirmed the localization of circPPP6R3, 18S rRNA, and U6 were used as positive controls respectively for the cytoplasmic and nuclear sublocation. Scale bars, 50 μm. B The expression of circPPP6R3 in cytoplasmic and nuclear fractions was validated by qRT-PCR analysis. C The miRNAs predicted as the targets of circPPP6R3 were analyzed by qRT-PCR in the biotin-labeled circRNA probe pull-down assay. D The biotin-labeled circPPP6R3 probe could capture more circPPP6R3. E miR-1238-3p in circPPP6R3-knockdown ccRCC cells was detected by qRT-PCR analysis. F Schematic illustration of circPPP6R3 wild type and circPPP6R3 mutant type dual-luciferase reporter vectors, and dual-luciferase reporter assay was performed in the 293T cells to determine the direct binding relationship between circPPP6R3 and miR-1238-3p.

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