Fig. 7: circPPP6R3 upregulated CD44 via sponging to miR-1238-3p. | Cell Death & Disease

Fig. 7: circPPP6R3 upregulated CD44 via sponging to miR-1238-3p.

From: Circular RNA circPPP6R3 upregulates CD44 to promote the progression of clear cell renal cell carcinoma via sponging miR-1238-3p

Fig. 7

A, B qRT-PCR and Western Blotting were conducted to detect the relative expression of CD44 in ccRCC cells treated with siRNAs and overexpressing plasmids for circPPP6R3, mimics, and inhibitors for miR-1238-3p. C The circPPP6R3-knockdown-induced diminishment of CD44 was attenuated by miR-1238-3p inhibitors in ccRCC cells. D Representative IHC images of CD44 in ccRCC tissues as well as xenografts tumor tissues in animal studies. Scale bars, 50 μm. E GSEA was performed to identify the downstream pathway of CD44 in TCGA patients based on the high and low expression of CD44. F Correlation analysis of the protein level between CD44 and MMP9, Vimentin from the database in CPTAC. G, H MMP9 and Vimentin were downregulated in CD44-silencing or circPPP6R3-silencing ccRCC cells. I Schematic diagram illustrating the biological functions and mechanisms of the regulatory axis of circPPP6R3/miR-1238-3p/CD44 in ccRCC progression.

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