Fig. 5: miR-24-3p inhibition promotes Caspase 3/7 activation in vitro.

A SW480 intestinal epithelial cells transfected with either control or miR-24-3p inhibitor in the presence or absence of the cell death inducer staurosporine for 4 h were lysed in a buffer containing a luminescent caspase 3/7 substrate. Three independent experiments. Mean ± SEM. B SW480 cells transfected with either control mimic or miR-24-3p mimic (O/E) in the presence or absence of staurosporine for 4 h were lysed in a buffer containing a caspase 3/7 reactive luminescent buffer. Three independent experiments. Mean ± SEM. C SW480 cells transfected with either control or miR-24-3p inhibitor in the presence or absence of tumor necrosis factor (TNF) for 4 h were lysed in a buffer containing a luminescent caspase 3/7 substrate. Six independent experiments. Mean ± SEM. D YAMC (Young Adult Mouse Colon) non-transformed colonic epithelial cells were transfected overnight with a miR-24-3p inhibitor and caspase activity was measured 24 h later. Four independent experiments. Mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001.