Fig. 3: MiR-541-5p promotes proliferation, migration, and invasion but inhibited apoptosis of HepG2, SK-Hep1, and SMMC-7721 cell lines. | Cell Death & Disease

Fig. 3: MiR-541-5p promotes proliferation, migration, and invasion but inhibited apoptosis of HepG2, SK-Hep1, and SMMC-7721 cell lines.

From: CircMTO1 suppresses hepatocellular carcinoma progression via the miR-541-5p/ZIC1 axis by regulating Wnt/β-catenin signaling pathway and epithelial-to-mesenchymal transition

Fig. 3

HepG2, SK-Hep1, and SMMC-7721 cells were transfected with NC, miR-541-5p mimics, and miR-541-5p inhibitor. A–C Expression of miR-541-5p in HepG2, SK-Hep1, and SMMC-7721 cells. D–F Cell proliferation was detected in HepG2, SK-Hep1, and SMMC-7721 cells using CCK-8 assay. G–I Effect of miR-541-5p on migration and invasion were assessed using transwell migration and invasion assays; Scale bar, 200 μm. J–L Cells were stained with Annexin V-APC and PI, and the percentage of apoptotic cells was determined through flow cytometry. Data were presented as mean ± SD of three independent experiments. *P < 0.05; **P < 0.01.

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