Fig. 2: Overexpression of WTIP inhibits cell proliferation in AML cells.
From: WTIP upregulates FOXO3a and induces apoptosis through PUMA in acute myeloid leukemia
A Western blot analysis of WTIP expression in KG1a and MOLM-13 cells transfected with WTIP or control vector. B RT-PCR analysis of WTIP mRNA expression in KG1a and MOLM-13 cells transfected with WTIP or control vector. Data are represented as mean ± SD from three independent experiments (***P < 0.001). C Cell proliferation was detected by MTT assay in KG1a and MOLM-13 cells transfected with WTIP or control vector. Data are represented as mean ± SD from three independent experiments (***P < 0.001). D MTT assay of KG1a and MOLM-13 cells transfected with WTIP vector shows a decrease in the number of colonies compared with control vector cells. Scale bars, 50 μm. E Colony numbers were calculated and data are represented as mean ± SD from three independent experiments (***P < 0.001).
