Fig. 5: FOXO3 gene expression is transcriptionally regulated by WTIP.
From: WTIP upregulates FOXO3a and induces apoptosis through PUMA in acute myeloid leukemia
A MOLM-13 cells transfected with WTIP or control vector were treated with doxycycline for 48 h, and cell lysates were subjected to immunoprecipitation and immunoblotting with anti-WTIP and anti-FOXO3a antibodies, respectively. B FOXO3a-Luc reporter vector was transfected into HEK293 cells, either in the presence of WTIP or control vector, and luciferase activity was measured 48 h after transfection. Data are represented as mean ± SD from three independent experiments (*P < 0.05). C Confocal immunofluorescence analysis of FOXO3a localization in KG1a and MOLM-13 cells transfected with WTIP or control vector. Scale bars, 50 μm.
