Fig. 4: LncRNA HOXA-AS2 promotes Treg cell proliferation and immune tolerance to facilitate glioma progression via the miR-302a/KDM2A axis. | Cell Death & Disease

Fig. 4: LncRNA HOXA-AS2 promotes Treg cell proliferation and immune tolerance to facilitate glioma progression via the miR-302a/KDM2A axis.

From: HOXA-AS2 contributes to regulatory T cell proliferation and immune tolerance in glioma through the miR-302a/KDM2A/JAG1 axis

Fig. 4

LN229 and A172 cells were treated with sh-NC + oe-NC, sh-lncRNA HOXA-AS2 + oe-NC, sh-lncRNA HOXA-AS2 + oe-KDM2A, and sh-lncRNA HOXA-AS2 + miR-302a inhibitor. A KDM2A expression in LN229 and A172 cells determined by RT-qPCR. B Western blot analysis of KDM2A protein in LN229 and A172 cells. C LN229 and A172 cell proliferation detected by CCK-8 assay. D Ratio of CD4+CD25+Foxp3+ cells in CD4+ cells co-cultured with LN229 and A172 cells analyzed by flow cytometry. E IL-10, TGF-β, IFN-γ, and TNF-α levels in the LN229 and A172 cell supernatant determined by ELISA. *p < 0.05. Data were shown as the mean ± standard deviation from. Statistical comparisons were performed using one-way ANOVA with Tukey’s post hoc test when more than two groups were compared. Variables were analyzed at different time points using two-way ANOVA with Bonferroni post hoc test. Cell experiments were repeated three times.

Back to article page