Fig. 2: NFIC positively regulates transcription of miR-200b. | Cell Death & Disease

Fig. 2: NFIC positively regulates transcription of miR-200b.

From: Nuclear factor I-C disrupts cellular homeostasis between autophagy and apoptosis via miR-200b-Ambra1 in neural tube defects

Fig. 2

Changes in protein expression (A) and mRNA expression (B) of NFIC after transfection of the NFIC plasmid or the control vector in C17.2 neural stem cells. C RT-qPCR analysis of miR-200b levels in cells transfected with the NFIC plasmid or the control vector. Levels of NFIC protein (D) and mRNA (E) after transfecting the NFIC SiRNA or the control SiRNA into cells. F miR-200b levels after silencing NFIC with SiRNA in C17.2 cells. G A diagram showing the construct of the miR-200b promoter-luciferase reporter encompassing three binding motifs of TTGGCA. H The miR-200b promoter construct was cotransfected with the NFIC plasmid or the control vector into cells. Luciferase activity was assayed 48 h after transfection. I ChIP analysis of NFIC binding to the three conserved motifs in the promoter of miR-200b (S1, S2, and S3). J Luciferase activity was measured after cotransfection of the WT or Mut type of the miR-200b promoter with or without the NFIC plasmid in C17.2 cells. The results represent three repeated experiments. *P < 0.05 vs. control group.

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