Fig. 4: miR-200b rescues NFIC knockdown-induced autophagy. | Cell Death & Disease

Fig. 4: miR-200b rescues NFIC knockdown-induced autophagy.

From: Nuclear factor I-C disrupts cellular homeostasis between autophagy and apoptosis via miR-200b-Ambra1 in neural tube defects

Fig. 4

A, B Protein expression of LC3 in normal and ATRA-induced NTD embryos (n = 3). C, D Protein levels of Ambra1, LC3, and NFIC after C17.2 cells were transfected with the NFIC plasmid or the control vector. E, F Effect of NFIC SiRNA on the protein expression of Ambra1, LC3, and NFIC. G, H Ambra1, LC3, and NFIC protein expression in cells transfected with the NFIC SiRNA and the control mimic or the miR-200b mimic. I The fluorescence intensity of LC3 in cells transfected with the NFIC SiRNA and the control mimic or the miR-200b mimic, scale bar = 5 µm. Experiments were repeated three times. *P < 0.05 vs. control group.

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