Fig. 5: NFIC-miR-200b circuit triggers apoptosis in C17.2 cells. | Cell Death & Disease

Fig. 5: NFIC-miR-200b circuit triggers apoptosis in C17.2 cells.

From: Nuclear factor I-C disrupts cellular homeostasis between autophagy and apoptosis via miR-200b-Ambra1 in neural tube defects

Fig. 5

A, B Protein levels of apoptosis markers (Cleaved-caspase 3, Bax, and Bcl-2) in E9.5 NTD mouse embryos compared with the control group (n = 3). C, D Protein expression of Cleaved-caspase 3, Bax, Bcl-2, and NFIC estimated by western blot in cells transfected with the NFIC SiRNA or the control SiRNA. E, F Changes in Cleaved-caspase 3, Bax, Bcl-2, and NFIC proteins after transfection of the NFIC plasmid with or without the miR-200b inhibitor. Apoptotic cells are shown by representative confocal microscopic images of C17.2 cells cotransfected with the NFIC plasmid and the miR-200b inhibitor or control inhibitor, using the active caspase 3 assay, scale bar = 100 µm (G) and TUNEL assay (H, I), scale bar = 100 µm. Data from three replicate experiments are shown in the bar graphs. *P < 0.05 vs. control group.

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