Fig. 3: The role of MAP4K4 in the SOX6-induced autophagy.
From: MAP4K4 mediates the SOX6-induced autophagy and reduces the chemosensitivity of cervical cancer
A Schematic representation on two potential SOX6 double-binding sites in the promoter region of MAP4K4 gene. B The binding activity of SOX6 to the two potential double-binding sites in the promoter of MAP4K4 gene was analyzed by ChIP-PCR. C Dual-luciferase assay analysis on the effects of SOX6 in the transcriptional activity of wild-type (WT) and mutant (mut 1 and mut 1 + 2) MAP4K4 gene promoter in HeLa cells. D Western blotting analysis on the levels of MAP4K4 and LC3B proteins regulated by SOX6 in HeLa-HA-SOX6-tet cells that the expression of endogenous MAP4K4 was knocked down by transfecting siMAP4K4 or E treating with small molecule inhibitor, PF-06260933 (3 μM). α-tubulin protein was used as the internal control. F Representative confocal microscopy images and G relative number of GFP-LC3 puncta in HeLa-HA-SOX6-tet cells transfected with GFP-LC3 expression plasmid and treated with or without Dox (4 μg/mL) and/or PF-06260933 (3 μM) for 48 h. Data are mean ± SEM and at least 50 cells scored. (***P < 0.001, NS non-significant, one-way ANOVA and post hoc Tukey tests).
