Fig. 4: The role of MAP4K4 in the SOX6-induced activation of MAPK/ERK pathway and inhibition of PI3K-Akt-mTOR pathway.
From: MAP4K4 mediates the SOX6-induced autophagy and reduces the chemosensitivity of cervical cancer
A Western blotting analysis on the protein levels of key molecules within MAPK/ERK and PI3K-Akt-mTOR signaling pathways in HeLa-HA-SOX6-tet and HeLa-HA-SOX6ΔHMG-tet cells. B Western blotting analysis on the protein levels of key molecules within MAPK/ERK and PI3K-Akt-mTOR signaling pathways in HeLa-HA-SOX6-tet cells that the endogenous MAP4K4 was knocked down by transfecting siMAP4K4 or C treating with small molecule inhibitor, PF-06260933 (3 μM). D Western blotting analysis on the protein levels of key molecules within MAPK/ERK and PI3K-Akt-mTOR signaling pathways in HeLa-HA-SOX6-tet cells transfected with pCDH-MCS (CTRL) or pCDH-Flag-MAP4K4 expression plasmids under Dox (4 μg/mL) treatment for 48 h. E Western blotting analysis on the protein levels of key molecules within MAPK/ERK and PI3K-Akt-mTOR signaling pathways in HeLa cells transfected with pCDH-MCS (CTRL) or pCDH-Flag-MAP4K4 expression plasmids, respectively. α-tubulin protein was used as the internal control. F Representative confocal microscopy images of HeLa cells co-transfected with GFP-LC3 and pCDH-MCS (CTRL) or pCDH-Flag-MAP4K4 expression plasmids. Data are mean ± SEM and at least 50 cells scored. (*P < 0.05, Student’s t-test, two tails).
