Fig. 2: ATRA reduces the activity of mitochondria in sensitive NB4 cells. | Cell Death & Disease

Fig. 2: ATRA reduces the activity of mitochondria in sensitive NB4 cells.

From: Role of cardiolipins, mitochondria, and autophagy in the differentiation process activated by all-trans retinoic acid in acute promyelocytic leukemia

Fig. 2

Three independent cultures of retinoid-sensitive NB4 and retinoid-resistant NB4.306 cells were treated with vehicle (DMSO) or ATRA (1 μM) for 24, 48, 72 and 96 h. At the end of the treatment, cells were contemporaneously stained with Mito-Tracker red and Mito-Tracker green fluorescent dyes and subjected to FACS analysis. A The panel shows representative FACS plots obtained following 24 and 72 h of exposure to ATRA. As indicated, the black dots refer to cells treated with vehicle (DMSO), while the red dots refers to cells treated with ATRA. B The column diagrams illustrate the quantification of the data obtained by FACS analysis of the three cultures of NB4 and NB4.306 cells. The left diagrams show the levels of Mito-Tracker green fluorescence. The right diagrams show the values of Mito-Tracker red/green fluorescence (red fluorescence following normalization for the green fluorescence value). Each value is the mean + S.D. of three samples. *Significantly different relative to the corresponding vehicle treated controls (p < 0.05, unpaired Student’s t test) **Significantly different relative to the corresponding vehicle treated controls (p < 0.01, unpaired Student’s t test). The results shown are representative of three independent experiments producing similar results. C Three cultures of NB4 and NB4.306 cells were treated with vehicle (DMSO) or ATRA (1 μM) for 48 and 72 h. At the end of the treatment total cell homogenates were used to determine citrate synthase activity. Each value is the mean + S.D. of three samples run in duplicate (N = 6). The data are representative of two independent experiments producing similar results. D Mitochondria were isolated from NB4 and NB4.306 cells cultured in the presence of vehicle or ATRA (1 μM) for 48 h. Mitochondrial Complex I, Complex III, and Complex IV enzymatic activities were measured on five replicates and the results are expressed as the Mean + SD. The data are representative of two independent experiments providing similar results.

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