Fig. 2: MET decreases MGO-induced intracellular ROS generation. | Cell Death & Disease

Fig. 2: MET decreases MGO-induced intracellular ROS generation.

From: Metformin prevents methylglyoxal-induced apoptosis by suppressing oxidative stress in vitro and in vivo

Fig. 2

HUVECs were treated with MET (0, 0.1, 1, and 10 mM) for 2 h and then exposed to MGO (200 μM) for 1 h. A, B Then, the cells were stained with DCFH-DA, and the fluorescence intensity was measured at 488/525 nm using a microplate reader. Scale bar, 100 μm. C–F The levels of SOD, CAT, and GSH-Px and the MDA content were measured with the respective ELISA kits. G The MMP was assessed with the JC-1 probe. The fluorescence intensities of JC-1 monomers (490/530 nm) and JC-1 aggregates (525/590 nm) were measured using a microplate reader. The ratio of JC-1 aggregates/JC-1 monomers was calculated. H Ultrastructural alterations of mitochondria were detected by TEM. Scale bars: 500 nm. All data are shown as the mean ± SD of three independent experiments. ##p < 0.01 vs. Control, *p < 0.05 vs. MGO, **p < 0.01 vs. MGO.

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