Fig. 4: MET suppresses MGO-induced apoptosis and Akt dephosphorylation. | Cell Death & Disease

Fig. 4: MET suppresses MGO-induced apoptosis and Akt dephosphorylation.

From: Metformin prevents methylglyoxal-induced apoptosis by suppressing oxidative stress in vitro and in vivo

Fig. 4

HUVECs were pretreated with MET (10 mM), LY-294002 (LY, 50 μM, a PI3K inhibitor), and the vehicle control for 2 h, followed by stimulation with MGO for 24 h. A, B Cell apoptosis was analyzed by flow cytometry based on Annexin V-FITC and PI double staining. C, D Cell apoptosis was also examined by the TUNEL assay. Scale bars, 100 μm. Representative images of cell apoptosis are shown. E Ultrastructural alterations of mitochondria were detected by TEM. Scale bars: 500 nm. F, G Representative western blots of total cell lysates after immunoblotting and probing with antibodies against Nrf2 and HO-1, which were quantified by densitometry and calculated as ratios to β-Actin. The values (mean ± SD from three independent experiments) are relative to the control and are expressed as fold changes. ##p < 0.01 vs. Control, **p < 0.01 vs. MGO, &&p < 0.01 vs. MGO + MET.

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