Fig. 1: SHCBP1 is overexpressed in bladder cancer.

A Thirty-two SHC1-binding proteins are shown. B Heat map representation showing the members among 32 genes differentially expressed between 19 cases of bladder cancer and paired adjacent nontumor tissues from the TCGA dataset, fold change = 2, p < 0.05. C SHCBP1 expression in 19 cases of bladder cancer and paired adjacent tissues from the TCGA dataset. D SHCBP1 expression in the TCGA and GTEx databases. E SHCBP1 expression in three cohorts (Sanchez-Carbayo Bladder 2, Lee Bladder, and Dyrskjot Bladder 3) from the Oncomine database. F The expression of SHCBP1 in bladder cancer and normal tissues was detected by IHC. Representative images and IHC scores are shown, ***p < 0.001. G Representative IHC images of the subcellular location of the SHCBP1 protein. H RT-qPCR analysis of SHCBP1 expression in twenty cases of bladder cancer and normal tissues; *p < 0.05. I Immunoblotting analysis of SHCBP1 expression in eight cases of bladder cancer and normal tissues. N nonneoplastic bladder tissues, T tumor tissue, *p < 0.05. J, K RT-qPCR and immunoblotting analysis of SHCBP1 expression in three bladder cancer cell lines (5637, T24, and UMUC-3) and ureteral epithelial cells (SV-HUC-1). L Kaplan–Meier curves depicting RFS in high‑ and low‑SHCBP1 patients from TCGA dataset. M, N Kaplan–Meier analysis of the relationship between SHCBP1 expression and DSS in the GSE13507 and GSE32548 cohorts.