Fig. 3: SHCBP1 is involved in EGF-induced cell migration and invasiveness in bladder cancer.

A, B RT-qPCR and immunoblotting analysis showing SHCBP1 expression in the indicated cells transfected with shNC or shSHCBP1. **p < 0.01 and ***p < 0.001. C GSEA indicating that SHCBP1 expression was positively associated with tumor invasiveness (data from TCGA). D Scratch assays indicating the effect of SHCBP1 depletion on EGF-induced motility of T24 and UMUC-3 cells. *p < 0.05 and ***p < 0.001. E Transwell assays showing the effect of SHCBP1 depletion on EGF-induced migration and invasion of T24 and UMUC-3 cells. *p < 0.05, **p < 0.01 and ***p < 0.001. F H&E staining of both the tumor tissue and muscle tissue in the subcutaneous tumors xenografted with the indicated cells. Black arrows point to the direction of tumor invasiveness. G H&E staining of metastatic lesions in the lungs of mice injected with the indicated cells for 6 weeks. The number of metastatic nodules was calculated for each group (n = 6) in the panel. *p < 0.05.