Fig. 4: RACGAP1 is an interactive protein of SHCBP1. | Cell Death & Disease

Fig. 4: RACGAP1 is an interactive protein of SHCBP1.

From: EGF-induced nuclear translocation of SHCBP1 promotes bladder cancer progression through inhibiting RACGAP1-mediated RAC1 inactivation

Fig. 4

A Endogenous SHCBP1 was immunoprecipitated with an anti-SHCBP1 antibody, and silver staining was performed to visualize the proteins interacting with SHCBP1. B Distinct protein bands in the GEL were subjected to mass spectrometry, and the top ten interacting partners are shown. C The peptide map of the protein band marked by the arrow is identified as RACGAP1. D, E The endogenous interaction between SHCBP1 and RACGAP1 was detected by coimmunoprecipitation and immunoblotting in T24 and UMUC-3 cells. F The exogenous interaction between HA-SHCBP1 and Flag-RACGAP1 was assessed by coimmunoprecipitation and immunoblotting in 293 T cells. G Confocal fluorescence microscopy observing the colocalization of SHCBP1 and RACGAP1 in UMUC-3 cells. H, I Immunoprecipitation and immunoblotting analysis indicated that the N-terminal amino acid sequence (1 ~ 428) of SHCBP1 specifically interacted with RACGAP1.

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