Fig. 5: EGF enhances the interaction between SHCBP1 and RACGAP1.

A, B, C Immunoprecipitation and immunoblotting assays were conducted to evaluate the effect of EGF on the binding of SHCBP1 and RACGAP1 in 5637, T24, and UMUC-3 cells. HC: Heavy chain. D An IF assay was used to observe the subcellular location of SHCBP1 and RACGAP1 in 5637 cells in response to EGF. E The effect of erlotinib on the binding of SHCBP1 and RACGAP1 in UMUC-3 cells was examined using immunoprecipitation and immunoblotting assays. F IF assay was used to observe the subcellular location of SHCBP1 and RACGAP1 following erlotinib treatment.