Fig. 4: SNAI2 activates the Akt pathway by inhibiting PHLPP2. | Cell Death & Disease

Fig. 4: SNAI2 activates the Akt pathway by inhibiting PHLPP2.

From: Transcription factor SNAI2 exerts pro-tumorigenic effects on glioma stem cells via PHLPP2-mediated Akt pathway

Fig. 4

A The expression of MAGI3, HRAS, and PHLPP2 in TCGA, the x-axis indicates the sample type and gene name, and the y-axis indicates the expression value (N indicates normal samples, T indicates tumor samples). B The expression of PHLPP2, MAGI1, and HRAS in the presence of oe-SNAI2 or SNAI2-KD as measured by RT-qPCR. C The expression of PHLPP2, p-Akt level, p-mTORC level and mTORC protein expression in the presence of oe-SNAI2 or SNAI2-KD in GSCs as measured by Western blot assay. D The binding site between SNAI2 and PHLPP2 promoter as detected by ChIP. E The binding site between SNAI2 and PHLPP2 promoter as detected by DNA agarose gel electrophoresis. F The binding efficiency between SNAI2 and PHLPP2 promoter as examined by luciferase reporter gene assay. *p < 0.05 vs. GSCs treated with oe-NC or si-NC. Each cell experiment was repeated three times independently.

Back to article page